The gym door handle had the most bacteria. My question was which surface had the most bacteria. I predicted the toilet seat but it was the door handle which had an explosionof bacteria. I think the toilet seat is inaccurate becayse it had no bacteria and it even was used . I also did the toilet handle and gum under the table. The toilet handle only had one dot of bacteria and the gum had 3. And tothis conclusion don't forget to wash your hands to protect yourelf and others.